saci hf (New England Biolabs)
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saci hf
Saci Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/saci+hf/SacI-HF/pmc13137149-36-0-2
Average 96 stars, based on 1098 article reviews
Saci Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/saci+hf/SacI-HF/pmc13137149-36-0-2
Average 96 stars, based on 1098 article reviews
saci hf - by Bioz Stars,
2026-10
96/100 stars
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Generated:Article Title: Nucleic acids encoding chimeric polypeptides for library screening Article Snippet: The target DNA-bands were extracted at 97 bp from the gel using the Qiagen Gel Extraction Kit II according to the manufacturer's instructions. .. Both, the above generated library fragment and the vector containing the ribosome display construct, were double digested as follows: One reaction contained 400 ng of the library DNA fragment, 1 μL each of the restriction enzymes BamHI-HF and Article Title: Bacterial metabolic remodeling by convergent evolution unlocks nutrient availability after a host switch Article Snippet: For agr complementation, the whole agr operon encoding RNAIII was amplified with the native promoter and ribosomal binding site (table S6) using Q5 polymerase (NEB). .. Constructs were generated in the expression plasmid pALC2073 , digested with BamHI-HF and Plasmid Preparation:Article Title: Nucleic acids encoding chimeric polypeptides for library screening Article Snippet: The target DNA-bands were extracted at 97 bp from the gel using the Qiagen Gel Extraction Kit II according to the manufacturer's instructions. .. Both, the above generated library fragment and the vector containing the ribosome display construct, were double digested as follows: One reaction contained 400 ng of the library DNA fragment, 1 μL each of the restriction enzymes BamHI-HF and Article Title: Bacterial metabolic remodeling by convergent evolution unlocks nutrient availability after a host switch Article Snippet: For agr complementation, the whole agr operon encoding RNAIII was amplified with the native promoter and ribosomal binding site (table S6) using Q5 polymerase (NEB). .. Constructs were generated in the expression plasmid pALC2073 , digested with BamHI-HF and Construct:Article Title: Nucleic acids encoding chimeric polypeptides for library screening Article Snippet: The target DNA-bands were extracted at 97 bp from the gel using the Qiagen Gel Extraction Kit II according to the manufacturer's instructions. .. Both, the above generated library fragment and the vector containing the ribosome display construct, were double digested as follows: One reaction contained 400 ng of the library DNA fragment, 1 μL each of the restriction enzymes BamHI-HF and Article Title: Bacterial metabolic remodeling by convergent evolution unlocks nutrient availability after a host switch Article Snippet: For agr complementation, the whole agr operon encoding RNAIII was amplified with the native promoter and ribosomal binding site (table S6) using Q5 polymerase (NEB). .. Constructs were generated in the expression plasmid pALC2073 , digested with BamHI-HF and other:Article Title: The Helicobacter pylori TlpD cytoplasmic chemoreceptor requires an intact C-terminus for polar localization and function. Article Snippet: Quantification of bands was carried out using Fiji ImageJ software for normalization of peaks to the area of the full-length TlpD peak. Article Title: Seipin-defined ER sites undergo distinct enrichment of factors for the biogenesis of sterol ester rich lipid droplets Article Snippet: SacI-HF , Expressing:Article Title: Bacterial metabolic remodeling by convergent evolution unlocks nutrient availability after a host switch Article Snippet: For agr complementation, the whole agr operon encoding RNAIII was amplified with the native promoter and ribosomal binding site (table S6) using Q5 polymerase (NEB). .. Constructs were generated in the expression plasmid pALC2073 , digested with BamHI-HF and Polymerase Chain Reaction:Article Title: An optogenetics-compatible red fluorescent calcium indicator with negligible blue light photoactivation Article Snippet: Plasmid libraries were constructed using 2-fragment Gibson assembly of one vector backbone and one insert (NEBuilder HiFi DNA Assembly, NEB). .. The pDx vectors were digested with Article Title: The Helicobacter pylori TlpD cytoplasmic chemoreceptor requires an intact C-terminus for polar localization and function Article Snippet: .. The PCR products encoded PstI and SacI restriction sites, which were digested with PstI-HF (NEB) and In Silico:Article Title: A Genetic Method for Distinguishing Cryptic Pocillopora Species in French Polynesia without Sequencing Article Snippet: .. A total of eight candidate enzymes were identified for further testing in silico : AciI (New England Biolabs (NEB) #R0551S, MA, USA), BanI (NEB #R0118S), BceAI (NEB #R0623S), BseYI (NEB #R0635S), EcoRV-HF (NEB #R3195S), HgaI (NEB #R0154S), NlaIV (NEB #R0126S), and |
